Thursday, June 21, 2012

Biotechnology and Genetic Disruptions


From The Academics Review Criticism of 'Genetic Roulette'

 “It is certainly true that crop breeding changes DNA; in fact, that is the purpose of all breeding programs—to create differences in DNA.  Campaigners who are opposed to GM crops consistently point to the potential harmful effects of DNA inserts, and the potential presence of multiple fragments of new DNA in a transformed plant.  There are several problems with their assertions: 1) All plant chromosomes are repeatedly disrupted by many structural changes to DNA that have occurred in plants repeatedly over the course of recent history, and still occur today when plants are grown each season in the field; 2) All breeding technologies produce numerous changes and disruptions to the structure of plant chromosomes; 3) Conventional breeding techniques, accepted as safe by all, cause much more genetic disruption than those introduced by genetic engineering, and the resulting plants are not tested extensively for genetic change nor for safety attributes using the rigorous standards applied to genetically engineered plants.”
 “Indeed, the use of more precise technology and the greater regulatory scrutiny probably make them even safer than conventional plants and foods; and if there are unforeseen environmental effects – none have appeared as yet – these should be rapidly detected by our monitoring requirements. On the other hand, the benefits of these plants and products for human health and the environment become increasingly clear.” ( European Commission 2001)

Read the full analysis: http://academicsreview.org/reviewed-content/genetic-roulette/section-2/2-1-dna-insertion/

References

Batista R and others (2008). Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion. Proceedings of the National Academy of Sciences of the United States of America 105(9): 3640–3645
.
Baudo MM, Lyons R, Powers S, Pastori GM, Edwards KJ, Holdsworth MJ, Shewry PR. (2006). Transgenesis has less impact on the transcriptome of wheat grain than conventional breeding. Plant Biotechnol J. 2006 Jul;4(4):369-80

Bejarano ER and others (1996). Integration of multiple repeats of geminiviral DNA into the nuclear genome of tobacco during evolution. Proc. Natl. Acad. Sci. U.S.A. 93:759– 764.
www.pnas.org/content/93/2/759.

Dooner HK and Weil CF (2007). Give-and-take: interactions between DNA transposons and their host plant genomes.  Current Opinion in Genetics & Development 2007, 17:486–492.

European Commission (2001). Press Release of 8 October 2001, announcing the release of 15 year study incl 81 projects/70M euros, 400 teams. (European Commission (2001). Press Release of 8 October 2001, announcing the release of 15 year study incl 81 projects/70M euros, 400 teams. European Commission (2001). Press Release of 8 October 2001, announcing the release of 15 year study incl 81 projects/70M euros, 400 teams. ec.europa.eu/research/fp5/eag-gmo.html and ec.europa.eu/research/fp5/pdf/eag-gmo.pdf

Fedoroff NV (1989). Maize transposable elements. In Mobile DNA. Editors Douglas E Berg and Martha M Howe.  American Society for Microbiology

Harper G and others (2002). Review. Viral sequences integrated into plant genomes. Annual Review of Phytopathology 40:119–36.

Jiang N and others (2004) Pack-MULE transposable elements mediate gene evolution in plants. Nature 431, 569-573.

Kashkush K and others (2002). Gene loss, silencing and activation in a newly synthesised a wheat allotetraploid. Genetics 160:1651-1659.

Kato A and others (2004). Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize.  Proceedings of the National Academy of Sciences of the USA 101(37): 13554-13559 www.pnas.org/content/101/37/13554

Kuiper HA, Kleter GA, Noteborn HPJM , and Kok EJ (2001). Assessment of the food safety issues relating to genetically modified food.The Plant Journal 27;6):503-526. Perhaps the best scientifically professional introduction to the topic of genetically modified food safety. Free access at www3.interscience.wiley.com/journal/118986104/abstract?CRETRY=1&SRETRY=0

Leitch AR ,Leitch IJ (2008). Genome plasticity and the diversity of polyploid plants. Science 320:481-483.

Lisch D. (2005). Pack-MULEs: theft on a massive scale. Bioessays 27:353-355.
Lough A and others (2008) Mitochondrial DNA transfer to the nucleus generates extensive insertion site variation in maize. Genetics, 178: 47-55 doi:10.1534/genetics.107.079624

McHughen A (2000). A Consumer’s guide to GM food : From Green Genes to Red Herrings . (Published as Pandora’s Picnic Basket in the USA). Oxford. Arguably the best book for the general reader about whether it is safe to eat the GM food.

Ming R and others (2008). The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus). Nature 252:991-997

Rutgers University Press Release (4th Oct 2006). Genome archaeology illuminates the genetic engineering debate. www.eurekalert.org/pub_releases/2006-10/rtsu-gai100306.php accessed Dec 11 2008. A Summary of  Bruggmann and others Genome Research 16:1241-1251.

Shirley BW and others (1992). Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations. The Plant Cell 4, 333-347.

Tanne E, Sela I (2005). Occurrence of a DNA sequence of a non-retro RNA virus in a host plant genome and its expression: evidence for recombination between viral and host RNAs.Virology.332(2):614-22.

Tribe D (2008). Blog posting. Gene-chips prove transgenes are clean genes. gmopundit.blogspot.com/2008/07/gene-chips-prove-transgenes-are-clean.html
Tribe D (2008 a). Blog posting. Safety, safety, safety, and more GM food safety. The Food and Chemical Toxicology Sextet. gmopundit.blogspot.com/2008/06/safety-safety-safety-and-more-gm-food.html.

Zabala G, Vodkin L.(2008). A putative autonomous 20.5 kb-CACTA transposon insertion in an F3′H allele identifies a new CACTA transposon subfamily in Glycine max. BMC Plant Biology. Research article Open Access www.biomedcentral.com/1471-2229/8/124.

1 comment:

Industry Analysis said...

very informative post which provide important information about how structural changes in DNA harmful for plant chromosome and how gentetic disruption occur..Thanx for providing this great post